Proteome Analysis of Recombinant CHO Cells Under Hyperosmotic Stress

  • 이문수 (한국과학기술원 생물과학과, 동물세포공학연구실) ;
  • 김경욱 (한국기초과학지원연구원 중앙분석기기부 프로테옴분석팀) ;
  • 김영환 (한국기초과학지원연구원 중앙분석기기부 프로테옴분석팀) ;
  • 이균민 (한국과학기술원 생물과학과, 동물세포공학연구실)
  • Published : 2003.04.11

Abstract

Under hyperosmotic stress, rCHO cells display decreased specific growth rate $({\mu})$ and increased specific antibody productivity $(q_{Ab})$. The effects of hyperosmotic stress on batch culture cellular dynamics are not well understood. To this end, we conducted a proteome profile of rCHO cells, using 2D-gel, MALDI-TOF-MS and MS/MS. As a result, the proteome profile of rCHO cells could be established using 41 identified proteins. Based on this proteome profile of rCHO cells, we have found at least 8 differently expressed spots at hyperosmotic osmolality (450 mOsm/kg). Among these spots, two metabolic enzymes were found to be up-regulated (pyruvate kinase and GAPDH), while down-regulated protein was identified as tubulin. It shows that hyperosmotic stress can alter metabolic state, by up-regulated activities of two glycolysis enzymes, which could lead to activate the generation of metabolic energy. Tubulin expression was down-regulated, suggesting a reduction of cell division. Finally, the increased conversion energy could leads to improve overall productivity.

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