통계실험계획법을 통한 중요인자 선정에 의한 형질전환 담배세포에서 hGM-CSF 생산 증대 연구

Plackette-Burman method, one of the statistical experimental methods, was used for the screening of main factor for the enhanced production of hGM-CSF. A nine-runs Plackette-Burman design including one center point was the screening design selected for the initial set of experiments. The analysis of the data from the Plackette-Burman experiments showed main effect. Sucrose, nitrogen and temperature were determined to be main factors to enhance the production of hGM-CSF because the absolute values of main effect of sucrose, nitrogen and temperature were higher than the other factors. The production of hGM-CSF was improved when initial sucrose concentration was 50 g/L, initial nitrogen concentration was 90 mM and initial temperature was $20^{\circ}C$.

통계실험계획법 중 하나인 Plackette-Burman법을 사용하여 실험을 설계하였으며, 수행된 실험을 통하여 6가지 인자 중 main effect의 절대값이 높은 당, nitrogen, 온도를 최종 선정할 수 있었으며, main effect 값이 양의 값을 가지는 당과 nitrogen은 첨가 농도가 높을수록, 음의 값을 가지는 온도는 낮을수록 실험에 유리하다는 것을 확인할 수 있었다.