Proceedings of the KIEE Conference (대한전기학회:학술대회논문집)

The Korean Institute of Electrical Engineers (대한전기학회)
권호 표지

Fabrication of the temperature controllable microreactor for trypsin treatment

온도 조절이 가능한 트립신 전처리 반응침의 제작

  • Sim, Tae-Seok (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Lee, Kook-Nyung (School of Chemical Engineering, Seoul National University) ;
  • Joo, Hwang-Soo (School of Chemical Engineering, Seoul National University) ;
  • Kim, Dae-Weon (School of Mechanical and Aerospace Engineering, Seoul National University) ;
  • Kim, Byung-Gee (School of Chemical Engineering, Seoul National University) ;
  • Kim, Yong-Hyup (School of Mechanical and Aerospace Engineering, Seoul National University) ;
  • Kim, Yong-Kweon (School of Electrical Engineering and Computer Science, Seoul National University)
  • 심태석 (서울대학교 전기.컴퓨터 공학부) ;
  • 이국녕 (서울대학교 응용화학부) ;
  • 주황수 (서울대학교 응용화학부) ;
  • 김대원 (서울대학교 기계항공공학부) ;
  • 김병기 (서울대학교 응용화학부) ;
  • 김용협 (서울대학교 기계항공공학부) ;
  • 김용권 (서울대학교 전기.컴퓨터 공학부)
  • Published : 2003.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

In the research of proteomics, mass spectrometry analysis is the essential method for identification of the unknown proteins. Trypsin treatment for the sample preparation of mass spectrometry is the inevitable procedure[1]. However, sample preparation procedure is cumbersome and time consuming. To resolve these problems, Temperature controllable microreactor was designed and fabricated. It consists of metering chamber, micro channel, reaction chamber, platinum (Pt) thin film heater and a temperature sensor so that micro metering and mixture of reagent with temperature control can be done on the same chip. The total size of the fabricated microreactor was $37{\times}30{\times}1\;mm^3$ and the size of channel cross section was $200{\times}100{\mu}m^2$. PID temperature controller was realized using NI DAQ, PCI-MIO-l6E-1 board and LabVIEW program.

Keywords