Heterosigma akasiwo의 핵형분석을 통한 생활사 연구를 위한 DAPI이용 기법

The goals of this study is to elucidate life cycle and to detect genetic differences within a single species of Heterosigma akashiwo. To elucidate life cycle of H. akashiwo, have to study of benthic stage and vegetative cell. So we studied identification of H. akashiwo cyst. The relative contents of DNA in nuclei were determined in Heterosigma akashiwo. Different stages of the life history were obtained from culture and natural sediments, and examined by microfluorometry after staining with the DNA-specific fluorochrome 4'-6-dianudubi-2-phenylindole(DAPI). Large cells mainly in exponensial stage, while small cell, pre-encystment cells(\ulcorner\ulcorner), showed in the end of the late growth stage. Type of DNA content showed the different with growth stage. Usually the small cell has the high level of IOD.