Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
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Bioavailability of Clonazepam in human plasma using a simple HPLC

  • Lee, Dong-Kyu (Dept. of Pharmacology, Coll. of Pharmacy, Chung Ang University) ;
  • Jeong, Ji-Hoon (Dept. of Pharmacology, Coll. of Pharmacy, Chung Ang University) ;
  • Park, Joon-Hong (Dept. of Pharmacology, Coll. of Pharmacy, Chung Ang University) ;
  • You, Dae-Sik (Dept. of Pharmacology, Coll. of Pharmacy, Chung Ang University) ;
  • Sohn, Uy-Dong (Dept. of Pharmacology, Coll. of Pharmacy, Chung Ang University)
  • Published : 2003.10.01
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Abstract

We aimed at determining bioavailability of clonazepam, an anxiolytic drug, and developing a simple analysis in human blood using HPLC. A rapid and sensitive HPLC method was developed and validated using reverse-phase C18 column with retension time and limit of quantification of clonazepam being 2.58 min and 5hg/ml, respectively. Quantification was performed at 235 nm with p-hydroxybenzoic acid ethyl ester as internal standard. The method involved a simple extraction. In order to study blood level profile in time, sight volunteers were enrolled and orally took 6 mg clonazepam once. (omitted)

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