Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.10b
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- Pages.223.1-223.1
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- 2003
Quantitative Determination of Amygdalin Epimers from Armeniacae Semen by High Performance Liquid Chromatography.
- Koo, Ja-Yong (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
- Hwang, Eun-Young (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
- Lee, Je-Hyun (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
- Hong, Seon-Pyo (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University)
- Published : 2003.10.01
Abstract
D-Amygdalin and its conversion product, neoamygdalin, were clearly separated on reverse-phase column chromatography by an optimized eluent of 10 mM sodium phosphate buffer (pH 3.5) containing 8.5% acetonitrile. Linearity for analyzing D-amygdalin and neoamygdalin was observed in the range from 0.05 to 0.5 mM. The detection limits for D-amygdalin and neoamygdalin were ca. 5 uM per injected amount. When extracting amygdalin from a whole piece of Armeniacae Semen in the boiling aqueous solution, there was almost no influence of emulsin; it resulted in higher extraction yield. (omitted)
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