Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.10b
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- Pages.216.2-216.2
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- 2003
HPLC Analysis of Phytosphingosine and Its Metabolites in Mammalian Cells with TCPO-$H_2O$ $_2$ Chemiluminescence Reaction
- Jin, You-Xun (College of Pharmacy. Chungbuk National University) ;
- Choi, Chang-Hwan (College of Pharmacy. Chungbuk National University) ;
- Yoo, Hwan-Soo (College of Pharmacy. Chungbuk National University) ;
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Lee, Yong-Moon
(College of Pharmacy. Chungbuk National University)
- Published : 2003.10.01
Abstract
Sphingolipids has been known to induce apoptosis, cell proliferation, differentiation and migration in a variety of cell types. Recently, its phosphate form was suggested that they may act both as an agonist ligand to SlPRs and a second messenger in intracellular action. Phytosphingosine(PHS) is not easily detected due to trace component of cellular lipids in mammalian and human tissues while this is a major sphingolipid in yeast and plants. We therefore developed highly sensitive and reproducible analytical method for PHS and its phosphate by oxalic acid bis(2,4,6-tri-chlorophenyl) ester(TCPO)-hydrogen peroxide(H
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