Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.10b
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- Pages.160.3-161
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- 2003
Search for acetaldehyde trapping agents by using alcohol dehydrogenase assay
- Lee, Hyun-Joo (College of Pharmacy, Ewha Womans University) ;
- Lee, Kang-Man (College of Pharmacy, Ewha Womans University)
- Published : 2003.10.01
Abstract
Aldehyde and active form of free oxygen produced in alcohol metabolism in liver are the cause of liver cell damage. The main system of alcohol metabolism is composed of alcohol dehydrogenase(ADH), aldehyde dehydrogenase(ALDH) and cytochrome P4502E1. Alcohol dehydrogenase is reversible in alcohol metabolism. To block the backward reaction and enhance alcohol oxidation, acetaldehyde trapping agents were assayed. The assay was carried out by measuring decreasing NADH at 340nm, using acetaldcehyde and NADH as substrate and coenzyme respectively. (omitted)
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