Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.10b
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- Pages.145.3-146
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- 2003
Agrobacterium-mediated transformation of Eleutherococcus senticosus with the squalene synthases gene derived from panax ginseng
- Seo, Jin-Wook (Department of Biotechnology, Chung-Ang University) ;
- Jeong, Jae-Hun (Korea Ginseng Institute, Chung-Ang University) ;
- Han, Sung-Tai (Korea Ginseng Institute, Chung-Ang University) ;
- Lee, Hak-Sung (Korea Ginseng Institute, Chung-Ang University) ;
- Choi, Yong-Eui (Korea Ginseng Institute, Chung-Ang University) ;
- Shin, Cha-Gyun (Korea Ginseng Institute, Chung-Ang University)
- Published : 2003.10.01
Abstract
Transgenic Eleutherococcus senticosus plants were prepared by introducing the genes for squalene synthase (SQS), hygromycin phosphotransferase (HPT) and green fluorescent Protein (GFP) through Agrobacterium-mediated transformation. The enzyme, SQS, represents a putative branch point in the isoprenoid pathway capable of diverting carbon flow specifically to the biosynthesis of phytosterol and oleanolic acid. The full SQS gene was isolated from P. ginseng roots. Early globular embryo clusters developed from embryogenic callus were used as the explant source. (omitted)
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