Time-dependent Degradation of Polyphenol Oxidase in Perilla Fructescens Leaves

  • Kim, An-Keun (Sookmyung Women's Unviersity) ;
  • Kim, Yoo-Kyung (Seoul Metropolitan Government Reserach Institute of Public Health & Environment and Sookmyung Women's University)
  • Published : 2003.10.01

Abstract

Time-dependent PPO activity was determined at $4^\times$C and $30^\times$C. The result of activity determination, PPO extracted by phosphate buffer containing triton x-1l4(tPPO) was more stable than PPO by phosphate buffer(bPPO). The result of electrophoresis, at first a band was appeared at 48kd. After 1-3days a partial degrade band was appeared in bPPO and three partial degrade bands in tPPO. No activity band was appeared in PPOs at $30^\times$C and bPPO at $4^\times$C after 4 days. (omitted)

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