Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.10b
- /
- Pages.117.2-117.2
- /
- 2003
Different mechanisms mediate uptake of lead in a rat glial cell line
- Cheong, Jae-Hoon (Department of Pharmacy, Samyuk University) ;
- Tan, Tan Blendyl (Kennedy-Krieger Institute, Johns Hopkins Univ.) ;
- Kim, Y.B. (Kennedy-Krieger Institute, Johns Hopkins Univ.) ;
- Bannon, Bannon Desmond (Kennedy-Krieger Institute, Johns Hopkins Univ.) ;
- Olivi, Olivi Luisa (Kennedy-Krieger Institute, Johns Hopkins Univ.) ;
- Bressler, Bressler Joseph (Kennedy-Krieger Institute, Johns Hopkins Univ.)
- Published : 2003.10.01
Abstract
The mechanism by which lead enters glial cells was examined. The uptake of lead reached saturation when assays were performed in buffers at pH 5.5 and 7.4. The Vmax and Km was 2.7 pmoles/mg protein/min and 13.4 M in the buffer at pH 7.4, respectively, whereas the Vmax and Km was 329 fmoles/mg and 8.2 M in the buffer at pH 5.5, respectively. Uptake in a buffer at pH 5.5 but not at pH 7.4 was inhibited by iron. Cells treated with the iron chelator desferoxamine displayed higher levels of the divalent metal transporter mRNA and protein. (omitted)
Keywords