Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.10b
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- Pages.105.2-105.2
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- 2003
Role of the de novo Ceramide and Arachidonic Acid in Paclitaxel-Induced Apoptosis
- Chin, Mi-Reyoung (Department of Environmental & Health Chemistry, College of Pharmacy, Chung-Ang University) ;
- Kang, Mi-Sun (Department of Environmental & Health Chemistry, College of Pharmacy, Chung-Ang University) ;
- Kim, Dae-Kyong (Department of Environmental & Health Chemistry, College of Pharmacy, Chung-Ang University)
- Published : 2003.10.01
Abstract
Recently, several reports suggest that ceramide formation has been implicated in the apoptosis signaling in response to chemotherapeutic agents. In this study, to enhance paclitaxel-mediated cytotoxicity and endogenous ceramide levels, we blocked ceramide metabolism using an inhibitor of glucosylceramide synthase, l-phenyl-2- dacanoylamino-3-morpholino-l-propanol(PDMP) and SM synthase, D609. Exposure of human breast cancer cells to paclitaxel accumulated de novo ceramide synthesis by enhancement of SPT activity 1.2-fold, whereas ceramide synthase activity was not altered. (omitted)
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