Bioavailability of tolperisone in human plasma using a simple HPLC.

  • Jeong, Ji-Hoon (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Park, Joon-Hong (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Choi, Tae-Sik (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Lee, Dong-Kyu (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Jung, Chan-Heon (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Son, Byung-Hyuk (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Sohn, Uy-Dong (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
  • Published : 2003.04.01

Abstract

We aimed at determining bioavailability of tolperisone, a muscle relaxant. and developing a simple analysis in human blood using HPLC. A rapid and snsitive HPLC method was developed and validated using reverse-phase C18 column with retention time and limit of quantification of toferisone being 7.3 min and 20 ng/$m\ell$, respectively. Quantification was performed at 260 nm with chlorphenesin as internal standard. (omitted)

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