Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
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An effective immunoaffinity clean-up method for multi-DDT residue analysis

  • Hong, Ji-Youn (Korea Institute of Science ＆ Technology) ;
  • Hong, Jee-Eun (Department of Chemistry, Seoul Women′s University) ;
  • Lee, Eun-Ah (Department of Chemistry, Seoul Women′s University) ;
  • Park, Song-Ja (Department of Chemistry, Seoul Women′s University) ;
  • Lho, Dong-Seok (Department of Chemistry, Seoul Women′s University) ;
  • Kim, Jong-Hyun (Department of Chemistry, Seoul Women′s University) ;
  • Choi, Myung-Ja (Department of Chemistry, Seoul Women′s University)
  • Published : 2003.04.01
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Abstract

To increase detection sensitivity for multi-DDT residues (o,p-/p,p-DDT, o,p-/p,p-DDE, o,o-/o,p-DDD) analysis, a highly selective sample clean-up method was introduced prior to GC/MS analysis using immunoaffinity column. The immunoaffinity matrix was prepared by coupling IgG fraction of DDT antiserum to cyanogens bromide activated Sepharose 4B. Three DDT antisera (DDA-1, DDHP-2, DDCP-3) were test for affinity column ligand that obtained by imunizing respective DDT immunogen to rabbits, and IgG was purified using protein A affinity purification. (omitted)

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