Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
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Profiling Analysis of Sphingolipids in HL-60 Cells by High-Performance Liquid Chromatography-Tandem Mass Spectrometry in combination with Multiple Reaction Monitoring

  • Son, Jung-Hyun (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Lee, Jae-Ick (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technolog) ;
  • Yang, Ryung (Department of Biotechnology, Yonsei University) ;
  • Kim, Dong-Hyun (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology)
  • Published : 2003.04.01
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Abstract

Sphingolipid species are important second messengers due to their role in the mitogenesis, differentiation and apoptosis. We developed a new column liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS) in combination with multiple reaction monitoring (MRM) method for the rapid, simultaneous and quantitative determination of unambiguous detecting sphingolipids in cell culture of human cancer cells (HL-60). (omitted)

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