Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.04a
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- Pages.231.2-231.2
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- 2003
The efficient Erythropoietin expression system in Chinese Hamster Ovary cells by introduction of urea cycle enzymes
- Lee, Yun-Jeong (College of Pharmacy, Chung-Ang University) ;
- Kim, Jung-Kwon (College of Pharmacy, Chung-Ang University) ;
- Kim, Hyung-Jin (College of Pharmacy, Chung-Ang University) ;
- Kim, Na-Young (College of Pharmacy, Chung-Ang University) ;
- Kim, Jung-Hoe (Dept. of Biological Sciences, KAIS) ;
- Kim, Hong-Jin (College of Pharmacy, Chung-Ang University)
- Published : 2003.04.01
Abstract
The efficient EPO (Erythropoietin) expression system in Chinese Hamster Ovary (CHO) cells was devised through the removal of ammonium ion accumulated in the media by introducing urea cycle enzymes. Previously, we developed C05 cell by transfecting the carbamoly phosphate synthase (CPS) and ornithine transcarbamoylase (OTC) into the EPO expressing CHO cell, IBE. (omitted)
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