Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.04a
- /
- Pages.217.2-217.2
- /
- 2003
Why is $\beta$ -ketoacyl-ACP synthase II (FabF) is toxic in E. coli fatty acid biosynthesis\ulcorner
- Lee, Hee-Jung (Seoul Women′s University) ;
- Cho, Kyoung-Hea (Seoul Women′s University) ;
- Choi, Keum-Hwa (Seoul Women′s University)
- Published : 2003.04.01
Abstract
In the type II system. there are two elongation enzymes in E. coli, FabB is well-known to its ability to elongate cis-3-decenoly-ACP (C10:1) in unsaturated fatty acid synthesis, whereas FabF is important for the thermal regulation of fatty acid composition by its ability to elongate palmitoleic acid to vaccenic acid. based on their genetic mutation anaylsis. Radiochemical enzyme assay was performed using myristoyl-ACP as a substrate, which is known for general substrate of FabB and FabF. (omitted)
Keywords