Inhibition of lipopolysaccharide-induced inflammatory mediators NO, PGs, TNF-$\alpha$ expression by MeOH extract of Kochia scoparia in RAW264.7 cells.

  • Shin, Kyung-Min (Biochemistry Lab. College of Pharmacy, Kyunghee unv.) ;
  • Kim, Yang-Hee (Biochemistry Lab. College of Pharmacy, Kyunghee unv) ;
  • Park, Wan-Soo (Biochemistry Lab. College of Pharmacy, Kyunghee unv) ;
  • Lee, Kyung-Tae (Biochemistry Lab. College of Pharmacy, Kyunghee unv) ;
  • Park, Hee-Jun (Biochemistry Lab. College of Pharmacy, Kyunghee unv) ;
  • Choi, Jung-Won (Biochemistry Lab. College of Pharmacy, Kyunghee unv.)
  • Published : 2003.04.01

Abstract

MeOH extract obtained from the Kochia scoparia (KS) was observed to inhibit tumor necrosis factor-alpha (TNF-alpha), prostaglandins (PGs) and nitric oxide(NO) production in a lipopolysaccharide (LPS)-stimulated murine macrophage cell line, RAW 264.7. These effects of MeOH-KS were based on modulation of iNOS and COX-2 level. Western blot analysis showed that MeOH-KS reduced the iNOS and COX-2 level in LPS activated macrophages, in a dose dependent manner without cNOS and COX-1 protein level. (omitted)

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