Development of Nested RT-PCR for the Detection of Swine Hepatitis E virus in Formalin-fixed, Paraffin-embedded Tissues and Comparison with in situ Hybridization

  • Park, Jung-eun (Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Ha, Seung-kwon (Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Chae, Chan-hee (Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
  • Published : 2003.10.01

Abstract

Hepatitis E virus (HEV) has been recognized as a major cause of enterically transmitted non-A, non-B hepatitis in many developing countries. The taxonomy of HEV is not clear and the virus remains unclassified. The objective of this study was to optimize conditions and procedures to detect swine HEV in formalin-fixed, paraffin-embedded tissues by nested RT-PCR and compare this detection method with in situ hybridization. (omitted)

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