Proceedings of the Korean Society of Toxicology Conference (한국독성학회:학술대회논문집)

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Mutational profiling of p53 gene in human breast epithelial cells (MCF10A) exposed to Benzo(a)pyrene(BaP) or N-Methyl-N'-nitro-N-nitrosoguanidine(MNNG)

  • Kim, In-Sun (Division of Toxicology, College of Pharmacy, Sungkyunkwan University) ;
  • Lee, Byung-Mu (Division of Toxicology, College of Pharmacy, Sungkyunkwan University)
  • Published : 2003.05.01
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Abstract

Mutations of p53 tumor suppressor gene have been associated with exposure to carcinogens. Cultured human breast epithelial cells (MCF10A) were treated with Benzo(a)pyrene(BaP) or N-Methyl-N'-nitro-N-nitrosoguanidine(MNNG). MCF10A cells were grown in DMEM/F12 medium and trated for 6, 12, 24, 48 and 96h with Bap (1, 10 and 100 $\mu$M) dissolved in dimethylsulfoxide(DMSO). (omitted)

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