Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)
- 2003.04a
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- Pages.126-126
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- 2003
형질전환 벼에서 Cyanobacterial Sucrose-Phosphate Synthase 유전자의 발현
- Sang-Kyu Lee (Plant metabolism research center & Graduate school of biotechnology) ;
- Soo-Jung Lee (Plant metabolism research center & Graduate school of biotechnology) ;
- Na-Yeon Ryoo (Plant metabolism research center & Graduate school of biotechnology) ;
- Jang-Wook Lee (Plant metabolism research center & Graduate school of biotechnology) ;
- Seok-Yoon Yoon (Plant metabolism research center & Graduate school of biotechnology) ;
- Woon-Chul Shin (Plant metabolism research center & Graduate school of biotechnology) ;
- Se-Ho Ko (Plant metabolism research center & Graduate school of biotechnology) ;
- Deok Chun Yang (Department of Oriental Medicine Material and Processing, Kyung Hee Univ.) ;
- Youn-Hyung Lee (Plant metabolism research center & Graduate school of biotechnology)
- Published : 2003.04.01
Abstract
Sucrose-phosphate synthase (SPS) is a key regulatory enzyme in sucrose synthesis. To investigate the role of SPS in carbon partitioning, we produced transgenic rice plants overexpressing a cyanobacterial SPS from Synechocystis sp. PCC 6803. The gene was expressed under the control of the maize Ubil promoter in transgenic plants. Southern and Northern blot analyses confirmed the integration and the expression of the transgene in four transgenic rice lines. All of the four transgenic! lines analyzed showed abnormal vegetative and reproductive developments. Analysis of SPS activities and primary metabolites in the transgenic rice plants will be presented.
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