한국식물병리학회:학술대회논문집 (Proceedings of the Korean Society of Plant Pathology Conference)
- 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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- Pages.146.1-146
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- 2003
Virus-resistant and susceptible transgenic Nicotiana benthamiana plants expressing coat protein gene of Zochini green mottle mosaic virus for LMO safety assessment
- Park, M.H. (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University) ;
- B.E. Min (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University) ;
- K.H. Ryu (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University)
- 발행 : 2003.10.01
초록
Transgenic Nicotiana benthmiana plants harboring and expressing coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for both virus-resistant screening and complementation analysis of related viruses and environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N. benthamiana was performed using Agrobacterium-mediated method and the pZGCPPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and -susceptible lines, were obtained by screening of challenging homologous virus for T1 generations. Complementation of CP-deficient related virus was analyzed using the susceptible line of ZGMMV. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.
키워드