Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)

The Korean Society of Plant Pathology (한국식물병리학회)
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Detection of citrus-infecting viruses and sequence analysis of Satsuma dwarf virus(SDV) and SDV-CiMV in Jeju island

  • Lee, B.Y. (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University) ;
  • J.W. Hyun (Department of Agricultural Environment, National Jeju Agricultural Experiment Station, RDA) ;
  • Kim, K.S. (Department of Agricultural Environment, National Jeju Agricultural Experiment Station, RDA) ;
  • K.H. Ryu (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University)
  • Published : 2003.10.01
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Abstract

To investigate occurrence and variability of satsuma mandarin ( Citrus unshiu)-infecting viruses in Jeju island, several sets of diagnostic RT-PCR primers were designed and applied to samples collected randomly. Each primers set used in this survey was designed to detect Satsuma dwarf virus (SDV, Sadwavirus) and Citrus mosaic virus (CiMV) which is reclassified as an isolate of SDV (SDV-CiMV, Saduavirus). RT-PCR methods could detect SDV-CiMV and CTV from leaf . samples of unshui citrus. CTV was the prevalent and SDV-CiMV was not common in Jeju island. RT-PCR product of SDV-CiMV-JJl2 were cloned and sequenced. Sequence of the isolate revealed that it was 96.9 ％ identical to SDV-CiMV-Jp isolate at the nucleotide level. SDV-CiMV-JJl2 was propagated on Physalis floridana and sequencing of entire sequences of genome is in progress. Variability of SDV in Jeju island was confirmed by sequence comparisons and restriction mapping analysis.

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