Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)
- 2003.10a
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- Pages.145.1-145
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- 2003
Variability in the coat protein genes of two orchid viruses from Phlaenopsis orchids in Korea
- Park, S.H. (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University) ;
- H.R. Lim (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University) ;
- G.D. Ye (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University) ;
- K.H. Ryu (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University)
- Published : 2003.10.01
Abstract
This study was conducted to designing conserved regions of molecules for virus-derived resistance to transgenic Phlaenopsis orchids to protect against two major orchid viruses, Cymbidum mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV). Infected leaf samples of Phalaenopsis were randomly screened by the RT-PCR with specific primers to both of viruses. RT-PCR products of the viruses were cloned and their nucleotide sequences were determined. Multiple alignments of coat protein (CP) genes of the viruses revealed that over the 88 ％ and 94 ％ identities with CymMV and ORSV, respectively, were observed. These data can be useful for selection of highly conserved regions of CP gene of the viruses for transgenic orchid experiments.
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