한국식물병리학회:학술대회논문집 (Proceedings of the Korean Society of Plant Pathology Conference)

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지

Sensitive method for the detection of Apple scar skin viroid(ASSVd) by nested reverse transcription-polymerase chain reaction

  • Lee, Sung-Joon (Department of Microbiology, Kyungpook National University) ;
  • Kim, Chung (Department of Microbiology, Kyungpook National University) ;
  • Sim, Sang-Mi (Department of Microbiology, Kyungpook National University) ;
  • Lee, Dong-Hyuk (Daegu Apple Research Institute, National Horticultural Research Institute) ;
  • Lee, Jai-Youl (Department of Microbiology, Kyungpook National University)
  • 발행 : 2003.10.01
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초록

A rapid and sensitive assay for the specific detection of plant viroids using reverse transcription-polymerase chain reaction(RT-PCR) has been developed already. The nested RT-PCR assay cloud be applied for the detection of apple scar skin viroid(ASSVd) from young leaves and other tissues. ASSVd has central conserved region(CCR), terminal left(T$\sub$L/) and terminal right(T$\sub$R/) domain. Primers were designed from these regions. Primer sets were successfully applicable for the amplification of full length or partial region of ASSVd by nested RT-PCR. Nested RT-PCR assay was more sensitive and accurate method to detect ASSVd from young trees during the early time of apple cultivation.

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