Japanese Hornwort Mosaic Virus in Ornamental Flower and Its Phylogenetic Analysis with Other Potyvirusess.

  • Kim, Ok-Sun (National Seed Management Office, National Agricultural Research Center for Kyushu Okinawa Region, Kumamoto) ;
  • Ueda, S (National Agricultural Research Center for Kyushu Okinawa Region, Kumamoto) ;
  • Ebihara, Y. (Chiba Prefectural Agriculture Research Center, Chiba) ;
  • Uematsu, S. (Chiba Prefectural Agriculture Research Center, Chiba) ;
  • Hanada, K. (National Institute of Agribiological Resources, Tsukuba) ;
  • Ohshima, K. (Saga University) ;
  • Iwanami, T. (National Agricultural Research Center for Kyushu Okinawa Region, Kumamoto) ;
  • Takanami, Y. (Kyushu University) ;
  • Choi, Jang-Kyung (Kangwon National University)
  • Published : 2003.10.01

Abstract

Ammi majus (white lace flower, Unbelliferae) is an ornamental plant used for cut-flower arrangements worldwide. A potyvirus was isolated from its leaves with mosaic and chlorotic symptoms in the cultivated field of Chiba, Japan. Compared with Japanese homuort mosaic virus (JHMV) previously isolated from Cryptotaenia japonica, it showed similar characteristics in host reactions and molecular properties. The nucleotide sequences of coat protein and 3'- nontranslated region were highly homologous and shared 87% and 91% identities with those of JHMV, respectively. This virus was thus supposed to be an isolate of JHMV and designated as JHMV-Am. Phylogenetic tree was constructed using CP nucleotide sequences of the two isolates and other potyviruses previously reported. JHMV-Am and JHMV fell into a cluster with Korean strain of Zantedeschia mosaic virus (ZaMV-KR). However, low identity in amino acid sequences was found in the termini of CP genes between the two isolates of JHMV and ZaMV-KR.

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