Characterization of an Isometric virus Infecting Paprika (Capsicum annuum var. glosum) in Korea

  • Kim, Jae-Hyun (National Horticultual Research Institute, Rural Development Administration) ;
  • Kim, Jeong-Soo (National Horticultual Research Institute, Rural Development Administration) ;
  • Park, Gug-Seoun (National Horticultual Research Institute, Rural Development Administration) ;
  • Soo- Young Chae (National Horticultual Research Institute, Rural Development Administration) ;
  • Lee, Sin-Ho (National Horticultual Research Institute, Rural Development Administration) ;
  • Park, Jang-Kyung (Division of Biological Environment, Kangwon National University)
  • Published : 2003.10.01

Abstract

An Isometric virus was isolated from Paprika (Capsicum annuum var. glossum) showing necrosis spot and malformation on the fruit and the leaves, respectively, at yecheon in Korea. The virus could infect locally on Chenopodium amaranticolr, C. quinoa, Petunia x hybrida and Nicotiana glutinosa, but could not infect on Gomphrena globosa and Physalis floridana. The virus could infect systemically on red pepper and Lycopersicon esculentum. Datura stramonium, N. cleuarandii, N. rustim and N. tabacum cvs. were produced necrosis or necrotic ring spot lesions on the inoculated leaves and mosaic, vein necrosis or lethal death on the upper leaves. The virus was not related serologically to cucumber mosaic virus (CMV). In RT-PCR assay, it could not detected with specific primers of CMV and BBWV-II. The virions contain one molecule of genomic RNA, Which was approximately 3.8Kb and the coat protein (CP) of the purified virion migrated as a single band with molecular wight of about 29KDa in SDS-PAGE.

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