Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)
- 2003.10a
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- Pages.139.1-139
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- 2003
Improved RNA extraction for fruit tree viruses in RT-PCR assay
- Lee, Sin-Ho (National Horticultural Research Institute, Rural Development Administration) ;
- Kim, Hyun-Ran (National Horticultural Research Institute, Rural Development Administration) ;
- Kim, Jae-Hyun (National Horticultural Research Institute, Rural Development Administration) ;
- Kim, Jeong-Soo (National Horticultural Research Institute, Rural Development Administration)
- Published : 2003.10.01
Abstract
Tissues from woody plant contain higher amount of phenolic compounds and polysaccharides, which give inhibitory effects on reverse transcriptase and/or Taq ploymerase. The common multiple-step protocols using several additives to inhibit polyphenoic compounds during nucleic acid extraction are time consuming and laborious. Sodium sulfite (Na
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