Identification of Potato mop-top virus from Solanum tuberosum cv. Gawon in Korea

  • Lee, Young-Gyu (National Alpine Agricultural Experiment Station, RDA) ;
  • Park, Jong-A (National Alpine Agricultural Experiment Station, RDA) ;
  • Yoon, Young-Nam (National Alpine Agricultural Experiment Station, RDA) ;
  • Cheon, Jeong-Uk (National Alpine Agricultural Experiment Station, RDA) ;
  • Lee, Key-Woon (Department of Agricultural Biology, Kyungpook National University)
  • Published : 2003.10.01

Abstract

Potato mop-top virus(PMTV) was identified from Solanum tuberosum cv. Gawon showing bright chlorotic mottle symptom in Namwon, Korea. Samples were collected green-house in February, 2003. Electron microscopic examination of negatively stained preparation revealed that PMTV were rigid-rod shaped particles about 100-150, 250-300 nm x 18-20 nm in length. In ultrathin sections of leaf tissue from diseased potato plants, cluster of viruses particles were observed in the cytoplasm. TAS-ELISA determined that the virus was serologically related to PMTV. PMTV produced double ring necrotic local lesion in inoculated leaf of Chenopodium amaranticolor in incubated at 15$^{\circ}C$. The PMTV could be detected with RT-PCR using PMTV detectable primer set designed to amplify about 540 bp of the partial CP gene of PMTV

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