Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)
- 2003.10a
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- Pages.135.1-135
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- 2003
Detection of Xanthomonas axonopodis pv. citri, the causal agent of bacterial canker on Unshiu orange fruits using bacteriophage in Korea.
- Myung, Inn-Shik (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
- Lee, Young-Hee (Horticultural Environment Division, National Horticultural Research Institute)
- Published : 2003.10.01
Abstract
A technique for detection of Xanthomonas axonopodis pv. citri, a causal bacterium of canker on Unshiu orange fruits, was developed using bacteriophage. Procedure for the detection was designed on the basis of the previous reports that one group(CPI) of X. axonopodis pv. citri bacteriophage and corresponding two Iysotypes distributed in Korea. First, fruit surface was washed with sterile distilled water and pellet was obtained from centrifugation. The pellet was resuspended in Wakimoto's potato semi-synthetic broth medium and divided equally into two parts. One part was heated in boiling water to kill bacterial cells. Bacteriophages(CP
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