Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)
- 2003.10a
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- Pages.112.1-112
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- 2003
Analysis genetic diversity of Plasmodiophora brassicae using RFLP and RAPD(oral)
- Heo, Seung-Hwan (College of Agriculture and Life Sciences, Chungnam National University) ;
- Jang, Chang-Soon (College of Agriculture and Life Sciences, Chungnam National University) ;
- Lee, Hyoun-Kyoung (College of Agriculture and Life Sciences, Chungnam National University) ;
- Lee, Woo-Chung (College of Agriculture and Life Sciences, Chungnam National University) ;
- Jang, Se-Jeong (College of Agriculture and Life Sciences, Chungnam National University) ;
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Kim, Hong-Gi
(College of Agriculture and Life Sciences, Chungnam National University)
- Published : 2003.10.01
Abstract
Genetic diversity of Plasmodiophora brassicae from major chinese cabbage cultivating areas in Korea was analyzed by using PCR-RFLP and RAPD. Single spores of P brassicae isolated from galls of club root made induce lesion on chinese cabbage successfully. The PCR-RFLP and RAPD by primers PbITS, URP 3, 6 and OPA 7 revealed that single spore isolates showed various DNA polymorphisms among them unrelated geographic origins. These results indicate that P. brassicae population in Korea showed genetic difference among them. This study could be facilitate to identify genetic characteristics ofP. brassicae based on DNA polymorphisms between single spore isolates and to get basic information which can be used to advanced resistance breeding against club root of chinese cabbage.
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