Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)
- 2003.10a
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- Pages.97.1-97
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- 2003
Identification of an Antagonistic Bacterium, KJ1R5, for Biological Control of Phytophthora Blight of Pepper
- Kim, Hye-Sook (Collage of Life and Environmental Sciences, Korea University) ;
- Myung, Inn-Shik (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
- Kim, Ki-Deok (Collage of Life and Environmental Sciences, Korea University)
- Published : 2003.10.01
Abstract
An antagonistic bacterium, KJ1R5,, to Phytophthora capsici was obtained from root interior of a healthy pepper plant. To identify the bacterial antagonist, 16S rDNA sequence analysis, Biolog system, fatty acid methyl-esters (FAMEs), and physiological and biochemical characterization were conducted. The determined 165 rDNA sequence of KJ1R5, showed higher similarities to those of a group consisting of several Chryseobacterium strains with 95.2, 95.2, and 95,1% similarity to C. defluvii, Chryseobacterium sp. FR2, and C. scophthalmum, respectively, In addition, Halounella gailinarum, Bergeyella zoohelcum, and Riemerella anatipestifer are another group for KJ1R5, with 94.1, 89.7, and 87.2% similarities, respectively When identification of the antagonistic bacterium, KJ1R5, was conducted using BIOLOG system, the strain KJ1R5, was identified as Flavobacterium tirrenicum (similarity; 0.75%). Fatty acid profiles of the strain KJ1R5, were composed mainly of iso-17:0 w9c and iso-15:0 and identified as Chryseobacterium balustinum (similarity 0.524%). KJ1R5, was Gram-negative, regular short rods ranging from 0.8
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