Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)
- 2003.10a
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- Pages.78.2-79
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- 2003
Differential expression and in situ localization of a pepper defensin (CADEFl) gene in response to pathogen infection, abiotic elicitors and environmental stresses in Capsium annuum
- Do, Hyun-Mee (Mushroom Experimental Station, Kyonggido Agricultural Research and Extension Services) ;
- Lee, Sung-Chul (College of Life and Environmental Sciences, Korea University) ;
- Jung, Ho-Won (College of Life and Environmental Sciences, Korea University) ;
- Hwang, Byung-Kook (Mushroom Experimental Station, Kyonggido Agricultural Research and Extension Services)
- Published : 2003.10.01
Abstract
Pepper defensin ( CADEFl) clone was isolated from cDNA library constructed from pepper leaves infected with avirulent strain Bv5-4a of Xanthomonu campestris pv. vesicatoria. The deduced amino acid sequence of CADEFl is 82-64% identical to that of other plant defensins. Putative protein encoded by CADEFl gene consists of 78 amino acids and 8 conserved cysteine residues to form four structure-stabilizing disulfide bridges. Transcription of the CADEF1 gene was earlier and stronger induced by X campestris pv. vesicatoria infection in the incompatible than in the compatible interaction. CADEF1 mRNA was constitutively expressed in stem, root and green fruit of pepper. Transcripts of CADEFl gene drastically accumulated in pepper leaf tissues treated With Salicylic acid (SA), methyl jasmonate (MeJA), abscisic acid (ABA), hydrogen Peroxide (H
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