Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)
- 2003.10a
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- Pages.76.2-77
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- 2003
Complete nucleotide sequences of an Rsv-resistance overcoming isolate of soybean mosaic virus.
- Park, Bong-Kum (Department of Biology ＆ Medicinal Science, Seoul Women′s University) ;
- Ahn, Hye-Jin (Department of Biology ＆ Medicinal Science, Seoul Women′s University) ;
- Yum, Hye-Jung (Department of Biology ＆ Medicinal Science, Seoul Women′s University) ;
- Lee, Jae-Hwa (Department of Biology ＆ Medicinal Science, Seoul Women′s University) ;
- Park, Chang-Won (Department of Biology ＆ Medicinal Science, Seoul Women′s University, Biomed RRC, Pai Chai University) ;
- Ryu, Ki-Hyun (Department of Horticultural Science, Seoul Women′s University)
- Published : 2003.10.01
Abstract
The complete nucleotide sequences of genomic RNA of an isolate of soybean mosaic virus (SMV-CN18), which has ability to overcome Rsv resistance of soybean, have been determined. A large open reading frame encodes a polyprotein of 3068 amino acids with a predicted Mr of 350 kDa. Based on comparison with the proposed cleavage site of other potyviral polyproteins, nine mature proteins are predicted as a following order, P1, HC-Pro, P3, CI, 6K, VPg, NIa, NIb and coat protein (CP). The mature proteins of the strain share various amino acid identity with known SMV-G2, -G7 and -N strain, with the greatest variability occurring in the P1 (91 ％, 88 ％, 96％)and the lowest variability in the CP (100 ％, 99 ％, 100 ％). In addition, 5' untranslated region determined by 5' RACE is much more various than any coding regions. Difference in amino acid sequences throughout the genome is discussed in relation to resistance and susceptibility of soybean cultivars to SMV-CNl8.
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