Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)
- 2003.10a
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- Pages.74.1-74
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- 2003
Characterization of Pathogenesis and Plant Defence-related Genes Against Potato virus X infection empolying Potato X virus expresssin vector
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Park, Mi-Ri
(School of Agricultural Biotechnology, Seoul National University) ;
- Kwon, Sun-Jung (School of Agricultural Biotechnology, Seoul National University) ;
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Kim, Kook-Hyung
(School of Agricultural Biotechnology, Seoul National University)
- Published : 2003.10.01
Abstract
Differential display (DD) of mRNA is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). Using DD-RT-PCR we obtained many genes that expressed differentially in healthy and PVX-infected Nicotiana benthamima, using total RNAs extracted from healthy and PVX-infected N. benthamiana plants. Three hundred and twenty-five DNA fragments isolated from DD-RT-PCR were cloned and sequenced for further characterization. Several host genes including SKPI-like protein, heat shock transcription factor and Avr9/Cf-9 rapidly elicited protein were selected to obtain full-length open reading frame and to characterize their potential involvement in virus disease development and/or host's defense against virus infection employing PVX-based expression vector. Transcrips from wild-type and clones containing each selected gene were inoculated onto N. benthamiana Levels of virus replication were confirmedby RT-PCR and RNA blot analysis, Expression profiles and potential role(s) of selected genes upon PVX infection will be discussed.
Keywords