Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2003.06a
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- Pages.66-66
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- 2003
Effects of Different Energy Substrates on Blastocyst Formation, Cell Number and ICM Proportion in Mouse Two Cell Embryos
- Park, Sung-Baek (Division of Life Resources, Daegu University) ;
- Park, Kee-Sang (Division of Life Resources, Daegu University, Department of OB/Gyn, Kyungpook National University Hospital) ;
- Lee, Taek-Hoo (Department of OB/Gyn, Kyungpook National University Hospital) ;
- Chun, Sag-Sik (Department of OB/Gyn, Kyungpook National University Hospital) ;
- Song, Hai-Bum (Division of Life Resources, Daegu University)
- Published : 2003.06.01
Abstract
The aim of this study was to investigate effect of different energy substrates on embryonic development of mouse embryos. Two cell embryos, recovered from ICR female mice (4 weeks old) at 44~52hrs after hCG injection (mated just after hCG injection), were cultured fur 72 hrs in the medium (MEM) supplemented with the three different energy substrates [glucose(G), pyruvate(P) and lactate(L)] and combinations (Control: 0 mM: group A: G 0.5; B: G 3.15; C: P 0.1; D: P 0.32; E: L 5.87; F: L 10.5; G: G0.5+P0.32+L10.5; H: G3.15+P0.1+L5.87; I: G0.5+P0.1+L5.87; J: G3.15+P0.32+L10.5). Blastocysts were stained differentially using PI and bisbenzimide. The 69.8% of the 2 cell embryos cultured in group F were developed the blastocysts. This was the highest (NS) than all other tested groups (44.2~62.8%). Blastocysts, cultured in the group E (60.4