Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2003.06a
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- Pages.56-56
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- 2003
Various Expression Pattern of Beta-catenin in the Preimplantation Stage of Porcine Embryos
- Han, Jee-Soo (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology) ;
- Koo, Deog-Bon (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology) ;
- Shin, Bo-Rami (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology) ;
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Lee, Kyung-Kwang
(Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology) ;
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Han, Yong-Mahn
(Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology)
- Published : 2003.06.01
Abstract
Beta-catenin is very important in early development including involvement in cell adhesion, cell signaling, and developmental fate specification. Cell-cell interaction is an important process during mammalian embryonic development. In preimplantation embryos, embryonic compaction is the process of increased cellular flattening and adhesion of junctional complexes and results in a polarized distribution. Beta-catenin is associated with embryonic compaction in mammals. Here, we examined the relationship between beta-catenin expression and compaction in porcine embryos derived from in vitro fertilization. First of all, we investigated beta-catenin expression in each embryonic developmental stage and also focused on expression pattern according to full, partial and non-compaction at morula stage. We used the immunocyto-chemical method in this research. To confirm compaction affects on the embryonic development, we compared between compaction and developmental rates to the blastocyst. The result showed that compaction and non-compaction rates were 14.6% and 63.8% at 4 days after IVF, respectively The developmental rates to the blastocyst and their total cell number were 50.9% vs 36.4% and 41.4