Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)

The Korean Society of Animal Reproduction (한국동물번식학회)
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Expression of the Antioxidant Enzyme and Apoptosis Genes in in vitro Maturation lin vitro Fertilization of Porcine Embryos

  • H. Y. Jang (Division of Animal Resources Science, Kanagwon National University) ;
  • H. S. Kong (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Park, K. D. (East West Kidney Disease Research Institute, School of Medicine, Kyung Hee University) ;
  • G. J. Jeon (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Lee, H. K. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • B. K. Yang (Division of Animal Resources Science, Kanagwon National University)
  • Published : 2003.06.01
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Abstract

The present study was conducted to determine the expression of the antioxidant enzyme(CuZn-SOD, Mn-SOD and GPX and apoptosis gene(caspase-3) for in vitro culture in in vitro maturation and in vitro fertilization(IVM/IVF) embryos in porcine. Porcine embryos derived from IVM/IVF were cultured in NCSU23 medium under 5% $CO_2$ in air at 38.5$^{\circ}C$. The patterns of gene expression for several antioxidant enzyme and apoptosis genes during preimplantion porcine embryo development were examined by the modified semi-quantitative single cell reverse transcriptase- polymerase chain reaction (RT-PCR). Preimplantation porcine embryos produced by IVM/IVF have expressed mRNAs for CuZn-SOD and GPX, whereas transcripts for Mn-SOD have not detected at any developmental stages. Expression of caspase-3 mRNA was detected at 2 cell, 8 cell, 16 cell and morula stages. The fas ligand transcripts were detected in porcine blastocyst. These results suggest that various antioxidant enzymes and apoptosis genes play crucial roles in in vitro culture of porcine IVM/IVF embryos.

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