한국동물번식학회:학술대회논문집 (Proceedings of the KSAR Conference)
- 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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- Pages.33-33
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- 2003
High Postnatal Survival and Efficacy of Female-Derived Donor Cells in the Productive of Somatic Cloned Piglets
- Cho, Seong-Keun (Major of Diary Science, Division of Applied Life Science, College of Agriculture) ;
- Park, Mi-Ryung (Major of Diary Science, Division of Applied Life Science, College of Agriculture) ;
- Hwang, Kyu-Chan (Major of Diary Science, Division of Applied Life Science, College of Agriculture) ;
- Kwon, Deug-Nam (Major of Diary Science, Division of Applied Life Science, College of Agriculture) ;
- Im, Yeo-Jeoung (Major of Diary Science, Division of Applied Life Science, College of Agriculture) ;
- Park, Ju-Joung (Major of Diary Science, Division of Applied Life Science, College of Agriculture) ;
- Son, Woo-Jin (College of Veterinary Medicine, Gyeongsang National University) ;
- Kim, Jin-Hoi (Major of Diary Science, Division of Applied Life Science, College of Agriculture)
- 발행 : 2003.06.01
초록
This study was conduct to compare the efficacy to produce male and female somatic cloned piglets. Maturation of porcine COCs was accomplished by incubation in NCSU-23 medium supplemented with 0.6 mM cysteine, 10% porcine follicular fluid, 1mM dibutyryl cyclic adenosine monophosphate (dbc-AMP, Sigma, USA), and 0.1 IU/ml human menopausal gonadotrophin (hMG, Teikokuzoki, Japan) for 20h and then cultured without dbcAMP and hMG for another 18 to 24 h. Female and male fetal cells were isolated from each fetus, cultured in ES-DMEM medium containing 10% FCS. Enucleated oocytes were fused with fetal fibroblasts (passage 4 to 15). Reconstructed embryos were cultured in NCSU-23 with 4 mg/ml BSA under mineral oil at 39