초록
The combined effect of bioaugmentation of dechlorinating bacterial cultures and addition of iron powder (Fe$^{0}$ ) on reductive dechlorination of tetrachloroethylene (PCE) and other chlorinated ethylenes in a artificially contaminated soil slurry (60$\mu$mo1es PCE/kg soil) were tested. Two different anaerobic bacterial cultures, a pure bacterial culture of Desulfitobacterium sp. strain Y-51 capable of dechlorinating PCE to cis-1, 2-dechloroethylene (cis-DCE) and the other enrichment culture PE-1 capable of dechlorinating PCE completely to ethylene, were used for the bioaugmentation test. Both treatments introduced with the strain Y-51 and PE-1 culture (3mg dry cell weight/kg soil) showed conversion of PCE to cis-DCE within 40 days. The treatments added with Fe$^{0}$ (0.1 -1.0 %(w/w)) alone to the soil slurry resulted in extended PCE dechlorination to ethylene and ethane and the, dechlorination rate depended on the amount of Fe$^{0}$ added. The combined use of the bacterial cultures with Fe$^{0}$ (0.1-1.0%) showed the higher PCE dechlorination rate than the separated application and the pattern of PCE dechlorination and end-product formation was different from those of the separated application. These results suggested that the combined application of Fe$^{0}$ and the bactrial culture, specially the complete dechlorinating enrichment culture such as PE-1 culture, would be practically effective for remediation of PCE contaminated soil.
