Proceedings of the Korean Society of Developmental Biology Conference (한국발생생물학회:학술대회논문집)
- 2003.10a
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- Pages.128-128
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- 2003
Sow Transfer of Cultured Embryos : Embryo Recovery, Oocyte Collection, IVM-IVF-IVC Treatment, Vitrification/Thaw, and Surgical and Nonsurgical Transfer
- Kim, In-Doc (Department of Animal Science, Dankook University) ;
- Ahn, Mi-Hyun (Department of Animal Science, Dankook University) ;
- Hur, Tae-Young (Department of Livestock Improvement, National Livestock Research Institute, Seonghwan) ;
- Son, Dong-Soo (Department of Livestock Improvement, National Livestock Research Institute, Seonghwan) ;
- Hong, Moon-Pyo (Moonseong Pig Farm) ;
- Seok, Ho-Bong (Department of Animal Science, Dankook University)
- Published : 2003.10.01
Abstract
The aims of this study are 1) to test oocytes and embryos collected from in-vivo and in-vitro to achieving the valuable protocol by culturing, vitrifying and thawing of oocytes/embryos, and 2) to transfer them to recipient, and finally have resulted in pregnancies from recipient females after surgical or nonsurgical transfer. In vitro maturation and fertilization were performed according to the procedures of Funahashi et al. Fertilized oocytes were cultured in glucose-free NCSU 23 supplemented with 5 mM sodium pyruvate, 0.5 mM sodium lactate and 4 mg/ml bovine serum albumin for 2 days at 39