한국발생생물학회:학술대회논문집 (Proceedings of the Korean Society of Developmental Biology Conference)
- 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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- Pages.108-108
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- 2003
Survival Ability of Pig Embryos Frozen-Thawed by Open Pulled Straw Methods
- Lee, S.Y. (Biotechnology Division, Gyeongsangnam Province Advanced Swine Research Institute) ;
- Park, Y.H. (Biotechnology Division, Gyeongsangnam Province Advanced Swine Research Institute) ;
- D.S. Chung (Biotechnology Division, Gyeongsangnam Province Advanced Swine Research Institute) ;
- Park, C.K. (College of Animal Resources Sciences, Kangwon National University)
- 발행 : 2003.10.01
초록
The purpose of this is to investigate the effects of vitrification in open pulled straws (OPS) on in vitro survival of porcine embryos. Blastocysts were produced by in vitro fertilization of slaughterhouse-derived, in vitro matured oocytes with frozen-thawed boar semen, and subsequent culture on granulosa cell monolayer. After frozen-thawing, embryos were culture in NCSU-23 medium with 5 mM hypotaurine, 4 mg/