Proceedings of the Korean Society of Applied Pharmacology (한국응용약물학회:학술대회논문집)

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Determination of Prazosin in Human Plasma by a Validated HPLC Method with Application to Single-dose Pharmacokinetics

  • Youngah Cho (College of Pharmacy, Chosun University) ;
  • Junghyun Oh (College of Pharmacy, Chosun University) ;
  • Hyesun Gwak (College of Pharmacy, Chosun University) ;
  • Eunsun Noh (College of Pharmacy, Dongduk Women′s University) ;
  • Lee, Nayoung (College of Pharmacy, Dongduk Women′s University) ;
  • Inkoo Chun (College of Pharmacy, Dongduk Women′s University)
  • Published : 2003.11.01
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Abstract

A rapid and reproducible high performance liquid chromatographic assay of prazosin in human plasma was developed. After addition of internal standard (IS, terazosin hydrochloride) and alkalization of the plasma, the drug and IS were extracted into t-butylmethylether. The organic phase was back-extracted into 0.05% phosphoric acid and 50 ${\mu}\ell$ of the acid solution was injected into a reverse-phase C18 column with a mobile phase consisting of water : acetonitrile : triethylamine = 75 : 25 : 0.1 (pH 5.0). The samples were detected utilizing a fluorescence detector. A

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