한국자원식물학회:학술대회논문집 (Proceedings of the Plant Resources Society of Korea Conference)
- 한국자원식물학회 2003년도 심포지엄
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- Pages.59-67
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- 2003
High frequency plant regeneration from mature embryos of an elite barley cultivar (Hordeum vulgare L. cv Baegdong)
- Lee, Kui-Jae (Faculty of Bioresources Sciences, College of Agriculture, Chonbuk National University) ;
- Wtpsk, Senarath (Department of Botany, University of Sri Jayewardenepura) ;
- Lee, Wang-Hyu (Faculty of Bioresources Sciences, College of Agriculture, Chonbuk National University)
- 발행 : 2003.10.01
초록
An efficient plant regeneration system was developed for Hordeum vulgare L. cv Baegdong - an important Korean cultivar. The protocol was based on a series of experiments involving the sizes of mature embryos and the culture media. The embryo size is found to be critical for the establishment of embryogenic callus. Embryos of 1.1-1.5 mm size showed a much higher ability to produce embryogenic callus capable of regenerating green plants. The auxins picloram and dicamba proved effective in inducing callus from mature embryos.