한국생물공학회:학술대회논문집
- 한국생물공학회 2002년도 생물공학의 동향 (X)
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- Pages.453-456
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- 2002
반응표면 분석에 의한 Trichoderma sp. FJ1의 cellulolytic enzymes 생산의 최적화
초록
Trichoderma sp. FJl의 섬유소 분해효소 생산조건을 최적화하기 위해 반응표면 분석법을 이용하였다. 반응표면 분석을 위한 실험 계획법은 중심합성법 계획법을 이용하였으며, 주요 배양인자로서 탄소원의 농도, 질소원의 농도, 혼합탄소원 비율, 그리고 배양시간에 대해 조사하였다. CMCase의 경우에서는 탄소원의 농도 3.5%, 질소원 농도 0.6%, 혼합탄소원인 avicel 및 CMC의 비율 52:48, 그리고 배양시간 5.4일에 33.5 U/ml로 최적화되었고, xylanase의 생산은 3.5%, 0.8%, 54:46의 배양조건에서 5.3일에 52.6 U/ml이었고,
The production conditions of cellulolytic enzymes by Trichoderma sp. FJ1, were optimized using response surface analysis. The culture factors which largely affected to the production of enzymes such as cultivation time, carbon source concentration, nitrogen source concentration, and composition ratio of carbon sources were employed. Optimized conditions of the factors above to each cellulolytic enzyme production was as follow: CMCase production was obtained in the conditions of cultivation time of 5.4 days, 3.5% of carbon source concentration, 0.6% of nitrogen source concentration, and 52:48 (avicel:CMC) of composition ratio of carbon sources, respectively, xylanase appeared in the conditions of 5.3 day, 3.5%, 0.8%, and 54:46, respectively, and
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