자연계로부터 분리한 strain YJ에 의한 수소생산에 관한 연구

Hydrogen is considered to be a clean energy because it doesn't generate the global warming gas such as $CO_2$, SOx and NOx, after its combustion. In this study, strain YJ isolated from shore system was used to produce efficiently hydrogen using the various carbon sources such as glucose, sucrose and fructose and its characteristics were investigated in batch cultivation. The maximum hydrogen production shown that glucose, sucrose and fructose were highest obtained at 2, 4 and 5 % concentration, respectively. In addition, we investigated the effect of pH under various conditions as range of initial pH 5.5 to 8.0 pH because growth of strain YJ declined due to produced organic acids. The results showed that the highest production rate of hydrogen was obtained at pH 7.5.

Strain YJ는 혐기 발효에서 glucose를 이용하여 복합 유기산 및 수소를 생산하였다. 2% glucose를 이용할 때 총 38ml의 수소가 생산되었으며, pH 완충제인 인산염을 첨가시켰을 경우 1.21배의 수소가 증가되었다. 4% sucrose를 기질로 이용하였을 때 총 40.5ml의 수소를 생산하였으며, 5% fructose에서 38.1ml의 수소를 생산하였다. Glucose를 이용할 때 이 균주는 butyrate, propionate를 많이 축척하였고, acetate, formate등이 소량 검출되었다.