한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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조직공학을 이용한 각막상피 세포를 접종한 생인공간질층 제조

  • 안재일 (동국대학교 화학공학과) ;
  • 장인근 (동국대학교 화학공학과, 중앙대학교 부속용산병원 안과) ;
  • 김재찬 (중앙대학교 부속용산병원 안과) ;
  • 송계용 (중앙대학교 의과대학 병리학교실) ;
  • 이희구 (생명공학연구원 세포생물학연구실) ;
  • 윤도영 (생명공학연구원 세포생물학연구실) ;
  • 부하령 (생명공학연구원 세포생물학연구실) ;
  • 김기호 ((주)바이오랜드 생명공학연구소) ;
  • 박정극
  • Published : 2002.04.12
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Abstract

The corneal tissue consists of three layers : epithelium, stroma, and endothelium. Central cornea is a highly differentiated tissue whereas the limbus contains the epithelial stem cell. In the present study. we report the engineering of the three-dimensional reconstructed cornea derived from rabbit limbal epithelial and stromal cells. The differentiation degree of corneal stem cells were assessed in serum concentration and inoculation density of stromal cells. Optimal condition differentiation of corneal stem cells is achieved when 5% FBS was supplemented to culture medium and $1-2{\times}10^5$ cells/ml inoculation density of stromal cells.

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