한국생물공학회:학술대회논문집
- 한국생물공학회 2002년도 생물공학의 동향 (X)
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- Pages.271-272
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- 2002
Production of Newcastle Disease Virus by Vero Cell Culture
초록
Newcastle disease virus (NDV) vaccines were produced from Vero cells by using lively attenuated virus strain. The MOI of 0.1.' serum concentration of 2%. initial pH of 8.0. and infection time of 3 days were found to be optimum conditions for vaccine production. The treatment of polycation enhanced the virus production. When ascorbic acid was added as an antioxidant, NDV production was also enhanced. Utilization of
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