한국생물공학회:학술대회논문집
- 2002.04a
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- Pages.265-268
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- 2002
Development of Super-K562 Cells Producing Erythropoietin in Glucose-Free Medium
- Lee, Tong-Il (Graduate School of Biotechnology, Korea University) ;
- Choe, Yeon-Suk (Graduate School of Biotechnology, Korea University) ;
- Bae, Geun-Won (Graduate School of Biotechnology, Korea University) ;
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Kim, Ik-Hwan
(Graduate School of Biotechnology, Korea University)
- Published : 2002.04.12
Abstract
Lactate and ammonia are two major toxic waste products formed during mammalian cell culture. Accumulation of the side products have negative effects of on cell growth and specific production rate. In this study, K-562 cells were used as the host cell of a recombinant protein. Effects of carbon sources were invetigated focused on the cell culture span, the accumulation of lactate and ammonia in culture of recombinant K-562 cells.
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