한국생물공학회:학술대회논문집
- 한국생물공학회 2002년도 생물공학의 동향 (X)
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- Pages.261-264
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- 2002
Effect of HRE and Bcl-2 on the Production of Plasminogen Activator in CHO cells
- Bae, Geun-Won (Graduate school of Biotechnology, Korea University) ;
- No, Jeong-Gwon (Graduate school of Biotechnology, Korea University) ;
- Lee, Gyu-Min (Dept. Biological Science, KAIST) ;
- Kim, Ik-Yeong (Graduate school of Biotechnology, Korea University) ;
- Kim, Ik-Hwan (Graduate school of Biotechnology, Korea University)
- 발행 : 2002.04.12
초록
CHO (Chinese hamster ovary) cells were transfected with plasmids containing both cis-acting HRE (hypoxia response element) and CMV-promoter that controls tissue-type plasminogen activator (t-PA). CHO cells with HRE produced 16.2 fold higher t-PA concentration than CHO cells without HRE. It was noted that hypoxia strongly induced CHO cell apoptosis. which resulted in decrease of cell viability and protein production. In this study. by introducing Bcl-2, anti-apoptotic gene, we tried to recover cell viability and increase the protein production. When batch culture of both control cells without transfection of Bcl-2 and cells transfected with Bcl-2 were performed in the absence of CoCl ι hypoxia mimic condition. the cells with Bcl-2 were effected specific cell growth rates, maximum cell density. Immunoblotting assay showed Bcl-2 was recombinant with HRE dependent t- P A expression cassette, and their expression level was depended on hypoxia. By introducing Bcl-2, both cell viability and maximum cell density could be increased.
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